- 標(biāo)記技術(shù)
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labelling technique中文什么意思
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- By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis
通過采用gfp標(biāo)記技術(shù)，我們觀察到gfp - cam與胞質(zhì)分裂期細(xì)胞內(nèi)形成的特征結(jié)構(gòu)存在共分布。 - But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前對新發(fā)現(xiàn)的囊泡膜mu轉(zhuǎn)運體一dnn樣陽性終末與帶狀區(qū)內(nèi)pag樣陽性神經(jīng)元之間ej關(guān)系，以及谷氨酸脫發(fā)酶（ gad ，是gaba能神經(jīng)元和終末的特異性標(biāo)識物）是否參與其調(diào)控作用，尚缺乏系統(tǒng)的形態(tài)學(xué)資料。 - The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內(nèi)給予細(xì)菌內(nèi)毒素lps或腸毒素seb ，用免疫組織化學(xué)方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達(dá)，并采用雙標(biāo)記技術(shù)觀察了1型il刁受體陽性神經(jīng)元和加壓素及催產(chǎn)素表達(dá)的關(guān)系。 - The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software
方法根據(jù)mtdna控制區(qū)及其周圍區(qū)域的序列，設(shè)計多對引物，探索優(yōu)化擴(kuò)增體系，使擴(kuò)增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標(biāo)記技術(shù)對樣本進(jìn)行dna測序， sequencinganalysis3 . 4和seq ede軟件進(jìn)行序列分析和比對。 - The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結(jié)合以上創(chuàng)建的方法和免疫熒光流式細(xì)胞術(shù)，用k562細(xì)胞株可溶性分泌物(上清)對外周血單個核細(xì)胞( pbmc )進(jìn)行培養(yǎng)以模擬體內(nèi)微環(huán)境，然后分別從細(xì)胞增殖、凋亡、壞死、細(xì)胞周期、活性、細(xì)胞因子和表面抗原表達(dá)等方面進(jìn)行研究，結(jié)果發(fā)現(xiàn)用腫瘤上清培養(yǎng)的pbmc細(xì)胞數(shù)量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細(xì)胞數(shù)的下降主要是由細(xì)胞壞死和凋亡引起的，腫瘤上清對細(xì)胞周期沒有阻斷作用，反而略有促進(jìn)作用； t細(xì)胞亞群比例增加，并促進(jìn)表達(dá)th1 、 th2細(xì)胞因子。